A zygote generated by IVF with frozen/thawed ejaculated sperm and observed at 16 h post-insemination showing normal cytoplasmic morphology (400× magnification). NPBs are obviously different in the two PNs. It was transferred but failed to implant.
Figure 191
A zygote generated by ICSI, which subsequently underwent polar body biopsy, shows normal cytoplasmic morphology (400× magnification). It was discarded due to aneuploidy.
Figure 192
A zygote generated by IVF with frozen/thawed ejaculated sperm and observed at 16 h post-insemination showing normal cytoplasmic morphology with an evident clear cortical zone, the halo, in the cytoplasm (400× magnification). NPBs are scattered and different-sized, polar bodies are fragmented. It was cryopreserved.
Figure 193
A zygote generated by ICSI showing normal cytoplasmic morphology, a thin ZP and small debris in an enlarged PVS (400× magnification). It was transferred but clinical outcome is unknown.
Figure 194
A zygote generated by ICSI showing normal cytoplasmic morphology except for a refractile body visible at the 3 o'clock position in this view (400× magnification). It was transferred but clinical outcome is unknown.
Figure 195
A zygote generated by ICSI displaying heterogeneous, granular cytoplasm (200× magnification). NPBs are large-sized and polar bodies are fragmented. It was discarded due to poor subsequent development.
Figure 196
A zygote generated by IVF using frozen/thawed ejaculated sperm and observed at 17 h post-insemination showing an irregular oolemma and dysmorphic granular cytoplasm (400× magnification). PNs are different in size and peripherally located. NPBs differ in size and number between PNs. It was transferred but failed to implant.
Figure 197
A zygote generated by ICSI with peripheral PNs (150× magnification). The oolemma is irregular and the cytoplasm is dysmorphic and granular. The ZP is thick and dark. It was discarded.
Figure 198
A zygote generated by ICSI displaying granular cytoplasm, especially in the area immediately adjacent to the clear cortical zone (400× magnification). There is an enlarged PVS and an ovoid ZP. NPBs differ in number and size. It was transferred but clinical outcome is unknown.
Figure 199
A zygote generated by ICSI with four PNs (possibly a result of fragmentation of an originally normal-sized PN), displaying very granular cytoplasm and a clear cortical zone (600× magnification). It was discarded.
Figure 200
A zygote generated by ICSI using frozen/thawed ejaculated sperm (200× magnification). PNs are peripherally located with a large inclusion positioned directly below the PNs that is displaying a crater-like appearance as a consequence of severe organelle clustering. It was discarded.
Figure 201
An irregularly shaped zygote generated by ICSI showing centrally positioned PNs and a medium-sized vacuole at the 8 o'clock position in the cytoplasm (400× magnification). It was transferred but clinical outcome is unknown.
Figure 202
A zygote generated by ICSI with centrally positioned different-sized PNs, displaying two small vacuoles at the 9 o'clock position in the cytoplasm (400× magnification). It was transferred but clinical outcome is unknown.
Figure 203
A zygote generated by ICSI with an irregular oolemma and a large PVS with possibly highly fragmented polar bodies (150× magnification). Different sized overlapping PNs are peripherally positioned and several vacuoles of different sizes are present at the 2, 5 and 6 o'clock position in the cytoplasm. It was discarded.
Figure 204
A zygote generated by ICSI with slightly overlapping peripherally positioned PNs and a large PVS with one large and one fragmented polar body (150× magnification). Many small vacuoles are present throughout the cytoplasm. It was discarded.
Figure 205
Severely dysmorphic zygote generated by IVF showing small PNs and an irregularly shaped ZP and oolemma and lack of a PVS (600× magnification). There is a small vacuole present at 10 o'clock with refractile bodies immediately adjacent. It was discarded.
Figure 206
A zygote generated by ICSI showing peripherally positioned PNs and a very large vacuole at the 12 o′clock position in the cytoplasm (400× magnification). Polar bodies are fragmented and the ZP is of irregular thickness. It was discarded.
Figure 207
A zygote generated by ICSI showing cytoplasmic abnormalities (150× magnification). The PNs are juxtaposed and peripherally positioned with a large vacuole of irregular shape at the 6 o'clock position in the cytoplasm. The cytoplasm is granular and the ZP is thick and heterogeneous in appearance. It was discarded.
Figure 208
A zygote generated by ICSI showing severe cytoplasmic abnormalities (150× magnification). There is a large centralized vacuole with many small vacuoles surrounding it in a granular cytoplasm. It was discarded.
Figure 209
A zygote generated by ICSI showing peripherally located PNs with the same number and size of NPBs perfectly aligned at the PN junction (400× magnification). There is a large vacuole immediately adjacent to the two PNs that is almost the same size as the PNs. It was discarded.
Figure 210
A zygote generated by ICSI showing centrally positioned PNs and two large vacuoles immediately adjacent to each of the PNs at the 3 and 9 o'clock positions in the cytoplasm (400× magnification). There are also refractile bodies present at the 6–7 o'clock positions and an area of clustering at 11 o'clock. It was discarded.
